dNMP

Name: dNMP
Brand: WUHU HUAREN SCIENCE AND TECHNOLOGY CO., LTD.

dNMP, or deoxyribonucleoside monophosphate, is a building block of DNA. It consists of a deoxyribose sugar, a nitrogenous base (adenine, guanine, cytosine, or thymine), and a phosphate group. The nitrogenous base determines the specific nucleotide, and these nucleotides are paired with their complementary counterpart to form the rungs of the DNA ladder.

Types of Deoxyribonucleoside Monophosphate

dAMP·Free Acid
CAS NO. 653-63-4

dCMP·Free Acid
CAS NO. 1032-65-1

dGMP·Free Acid
CAS NO. 902-04-5

dTMP·Free Acid
CAS NO. 365-07-1

dUMP·Free Acid
CAS NO. 964-26-1

dIMP·Free Acid
CAS NO. 3393-18-8

dAMP·Na2
CAS NO. 2922-74-9

dCMP·Na2 CAS NO-13085-50-2
CAS NO-13085-50-2

dGMP·Na2
CAS NO. 33430-61-4

dTMP·Na2
CAS NO. 33430-62-5

dUMP·Na2
CAS NO. 42155-08-8

dIMP·Na2
CAS NO. 14999-52-1

The Role of dNMP in DNA Replication

Initiation: DNA replication begins at specific locations in the genome called origins of replication. Proteins bind to these origins and unwind the DNA to create a replication fork.

Primer Synthesis: An RNA primer is synthesized by primase. This primer provides a starting point for DNA synthesis.

Elongation: DNA polymerase enzymes add dNTPs to the growing DNA strand. The enzyme reads the template strand and incorporates the complementary dNTP. For example, if the template strand has an adenine (A), the polymerase will add a thymine (T) dNTP to the new strand.

Formation of Phosphodiester Bonds: When a dNTP is added to the growing DNA strand, it loses two of its three phosphate groups (as pyrophosphate), and a phosphodiester bond is formed between the 3' hydroxyl group of the last nucleotide in the chain and the 5' phosphate group of the incoming dNTP.

Proofreading: DNA polymerases have proofreading activity that checks and corrects any mismatched bases to ensure high fidelity in DNA replication.

Termination: Once the entire DNA molecule has been replicated, the process concludes, and the replication machinery disassembles.

How to Choose dNTP or dNMP from HUAREN?

Purity and Quality

Huaren Science's Standards: Both dNTPs and dNMPs from Huaren Science meet stringent purity standards, ensuring minimal contamination and high-quality results. Choose the product that aligns with your experimental needs while benefiting from Huaren Science's reputation for quality.

Application Requirements

dNTP: Ideal for DNA synthesis and PCR amplification. dNTPs are the building blocks for DNA polymerases during DNA replication and sequencing.

dNMP: Typically used in studies involving nucleotide metabolism and enzymatic reactions. dNMPs are the simplest form of nucleotides and are often used in biochemical assays.

Experimental Goals

High Fidelity DNA Replication: If your goal is to achieve high-fidelity DNA replication, such as in PCR or sequencing, Huaren Science's dNTPs are the best choice due to their high purity and reliability.

Metabolic Pathway Studies: For research focused on nucleotide metabolism or enzymatic activity, Huaren Science's dNMPs are more suitable.

Technical Support

Expert Guidance: As a leading China pharmaceutical intermediates supplier, Huaren Science offers excellent customer support and technical guidance. If you are unsure which product to choose, our experts can provide recommendations based on your specific research requirements.

Reach Out To Huaren Science!

If you have questions about our Amidites, Nucleotides or Nucleosides, feel free to contact Huaren Science's specialists for timely support!